pBI101.1/ProGOXL2:GUS
(Plasmid
#175558)
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PurposeBinary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175558 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepBI101.1
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Vector typePlant Expression
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Selectable markersKanamycin in plants
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameProGOXL2
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SpeciesA. thaliana (mustard weed)
- Promoter GOXL2
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Tag
/ Fusion Protein
- GUS (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBI101.1/ProGOXL2:GUS was a gift from George Haughn (Addgene plasmid # 175558 ; http://n2t.net/addgene:175558 ; RRID:Addgene_175558) -
For your References section:
Expression Patterns and Functional Characterisation of Arabidopsis GALACTOSE OXIDASE-LIKE (GOXL) Genes Suggest Specialised Roles for Galactose Oxidases in Plants. Sola K, Dean GH, Li Y, Lohmann J, Movahedan M, Gilchrist EJ, Adams KL, Haughn GW. Plant Cell Physiol. 2021 May 27. pii: 6286013. doi: 10.1093/pcp/pcab073. 10.1093/pcp/pcab073 PubMed 34042158