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pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct
(Plasmid #175549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct
  • Vector type
    Mammalian Expression, Mouse Targeting
  • Selectable markers
    Neomycin (select with G418) ; eGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WAPL
  • Species
    M. musculus (mouse)
  • Mutation
    none
  • Entrez Gene
    Wapl (a.k.a. A530089A20Rik, DIF-2, FOE, Wapal)
  • Tag / Fusion Protein
    • AID[71-114]-eGFP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct was a gift from Elzo de Wit (Addgene plasmid # 175549 ; http://n2t.net/addgene:175549 ; RRID:Addgene_175549)

  • For your References section:

    WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation. Liu NQ, Maresca M, van den Brand T, Braccioli L, Schijns MMGA, Teunissen H, Bruneau BG, Nora EP, de Wit E. Nat Genet. 2021 Jan;53(1):100-109. doi: 10.1038/s41588-020-00744-4. Epub 2020 Dec 14. 10.1038/s41588-020-00744-4 PubMed 33318687
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