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pTS26_QUAS_CsChrimson
(Plasmid #175548)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175548 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXL-BacII
  • Backbone manufacturer
    Olena Riabinina, Darya Task, Elizabeth Marr, Chun-Chieh Lin, Robert Alford, David A. O'Brochta & Christopher J. Potter
  • Backbone size w/o insert (bp) 6637
  • Total vector size (bp) 9233
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CsChrimson
  • Species
    Chloromonas subdivisa
  • Insert Size (bp)
    3289
  • GenBank ID
    KF992078
  • Promoter QUAS
  • Tag / Fusion Protein
    • tdTomato (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CTCGAGCAAAATGAGCAGACTGGTCGCCGCTTC
  • 3′ sequencing primer ATCCTCTAGAttacacctcgttctcgtagcagaaTTTATACAG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The CsChrimson plasmid p20X was shared by Vivek Jayaraman
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTS26_QUAS_CsChrimson was a gift from Leslie Vosshall (Addgene plasmid # 175548 ; http://n2t.net/addgene:175548 ; RRID:Addgene_175548)

  • For your References section:

    A persistent behavioral state enables sustained predation of humans by mosquitoes. Sorrells TR, Pandey A, Rosas-Villegas A, Vosshall LB. Elife. 2022 May 12;11:e76663. doi: 10.7554/eLife.76663. 10.7554/eLife.76663 PubMed 35550041
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