pTS26_QUAS_CsChrimson
(Plasmid #175548)

• Purpose: Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration
• Depositing Lab: Leslie Vosshall
• Publication: Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.7554/eLife.76663.

Backbone

• Vector backbone: pXL-BacII
• Backbone manufacturer: Olena Riabinina, Darya Task, Elizabeth Marr, Chun-Chieh Lin, Robert Alford, David A. O'Brochta & Christopher J. Potter
• Backbone size w/o insert (bp): 6637
• Total vector size (bp): 9233
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CsChrimson
• Species: Chloromonas subdivisa
• Insert Size (bp): 3289
• GenBank ID: KF992078
• Promoter: QUAS
• Tag / Fusion Protein:
  • tdTomato (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CTCGAGCAAAATGAGCAGACTGGTCGCCGCTTC
• 3′ sequencing primer: ATCCTCTAGAttacacctcgttctcgtagcagaaTTTATACAG

Resource Information

• Supplemental Documents:
  • pTS26_QUAS_CsChrimson.gb
• A portion of this plasmid was derived from a plasmid made by: The CsChrimson plasmid p20X was shared by Vivek Jayaraman
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry