Pomc-pGL3
(Plasmid #17553)

• Purpose: Luciferase expression under control of Pomc promoter (–646 to +65).
• Depositing Lab: Domenico Accili
• Publication: Kitamura et al Nat Med. 2006 May . 12(5):534-40.

Backbone

• Vector backbone: pGL3
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Pomc promoter
• Alt name: -646 to +65
• Species: M. musculus (mouse)
• Insert Size (bp): 708
• Entrez Gene: Pomc (a.k.a. ACTH, BE, Beta-LPH, Clip, Gamma-LPH, Npp, Pomc-1, Pomc1, alpha-MSH, alphaMSH, beta-MSH, gamma-MSH)
• Promoter: Pomc

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ? (unknown if destroyed)
• 3′ cloning site: ? (unknown if destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNRev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequence shows a C->T mutation in nt 129 of POMC. The depositing scientist is aware of this mutation and has found that it does not alter the promoter activity.