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pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9
(Plasmid #175525)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175525 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA5/FR/TO
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B-HaloTag10 and Tomm20-HaloTag9
  • Alt name
    H2B-HaloTag10
  • Alt name
    Tomm20-HaloTag9
  • Species
    H. sapiens (human)
  • Mutation
    HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag7-Q165H-P174R

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note there are an additional 35 amino acids at the end of the insert. Depositor confirms these do not affect plasmid function. This plasmid was used in the experiments discussed in their publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9 was a gift from Kai Johnsson (Addgene plasmid # 175525 ; http://n2t.net/addgene:175525 ; RRID:Addgene_175525)

  • For your References section:

    Engineered HaloTag variants for fluorescence lifetime multiplexing. Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K. Nat Methods. 2021 Dec 16. pii: 10.1038/s41592-021-01341-x. doi: 10.1038/s41592-021-01341-x. 10.1038/s41592-021-01341-x PubMed 34916672
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