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pET-51b-HaloTag10
(Plasmid #175520)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175520 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET51b(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HaloTag10
  • Alt name
    HaloTag10
  • Species
    H. sapiens (human)
  • Mutation
    HaloTag10 = HaloTag7-Q165W
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-51b-HaloTag10 was a gift from Kai Johnsson (Addgene plasmid # 175520 ; http://n2t.net/addgene:175520 ; RRID:Addgene_175520)

  • For your References section:

    Engineered HaloTag variants for fluorescence lifetime multiplexing. Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K. Nat Methods. 2021 Dec 16. pii: 10.1038/s41592-021-01341-x. doi: 10.1038/s41592-021-01341-x. 10.1038/s41592-021-01341-x PubMed 34916672
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