pGEX nsp1 R99A CoV2
(Plasmid
#175515)
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PurposeFor protein expression of R99A nsp1 CoV2
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175515 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX
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Backbone manufacturerGE (cytiva)
- Backbone size w/o insert (bp) 4950
- Total vector size (bp) 5490
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameR99A nsp1 CoV2
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Alt nameN-terminal mutant
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SpeciesCoV2
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Insert Size (bp)540
- Promoter tac promoter
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Tag
/ Fusion Protein
- GST tag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEX forward
- 3′ sequencing primer pGEX reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX nsp1 R99A CoV2 was a gift from Britt Glaunsinger (Addgene plasmid # 175515 ; http://n2t.net/addgene:175515 ; RRID:Addgene_175515) -
For your References section:
The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression. Mendez AS, Ly M, Gonzalez-Sanchez AM, Hartenian E, Ingolia NT, Cate JH, Glaunsinger BA. Cell Rep. 2021 Oct 19;37(3):109841. doi: 10.1016/j.celrep.2021.109841. Epub 2021 Sep 30. 10.1016/j.celrep.2021.109841 PubMed 34624207
