DDX41-GFP
(Plasmid #175494)

• Purpose: Total RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.
• Depositing Lab: Evan Braunstein
• Publication: Li et al Haematologica. 2016 Jun;101(6):e228-31. doi: 10.3324/haematol.2015.139790. Epub 2016 Mar 4.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6217
• Total vector size (bp): 7981
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DDX41
• Species: H. sapiens (human)
• Insert Size (bp): 1866
• GenBank ID: NM_001321732.2
• Entrez Gene: DDX41 (a.k.a. ABS, MPLPF)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: Age1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: EGFP-N reverse primer

Resource Information

• Supplemental Documents:
  • DDX41gfp.gbk
  • DDX41gfp.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry