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Addgene

XX01CAPN1A-c001
(Plasmid #175491)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175491 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)-TEV
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAPN1 / CAPNS1
  • Alt name
    CANPL1
  • Species
    H. sapiens (human)
  • Entrez Gene
    CAPN1 (a.k.a. CANP, CANP1, CANPL1, SPG76, muCANP, muCL)
  • Tags / Fusion Proteins
    • His6-TEV (N terminal on insert)
    • AviTag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Bicistronic for CAPN1 and CAPNS1. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XX01CAPN1A-c001 was a gift from Opher Gileadi (Addgene plasmid # 175491 ; http://n2t.net/addgene:175491 ; RRID:Addgene_175491)
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