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Addgene

FLAG-Foxo4
(Plasmid #17549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322-SV40
  • Backbone size w/o insert (bp) 3102
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FoxO4
  • Alt name
    forkhead box O4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1518
  • Entrez Gene
    FOXO4 (a.k.a. AFX, AFX1, MLLT7)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BglII (unknown if destroyed)
  • 5′ sequencing primer CCCCATGGCTGACTAATTTTT
  • 3′ sequencing primer EBV-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-Foxo4 was a gift from Domenico Accili (Addgene plasmid # 17549 ; http://n2t.net/addgene:17549 ; RRID:Addgene_17549)
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