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Addgene

PRDX1A-c001
(Plasmid #175473)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175473 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC-Bio3
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRDX1
  • Alt name
    MSP23|NKEF-A|NKEFA|PAG|PAGA|PAGB|PRX1|PRXI|TDPX2
  • Species
    H. sapiens (human)
  • Entrez Gene
    PRDX1 (a.k.a. MSP23, NKEF-A, NKEFA, PAG, PAGA, PAGB, PRX1, PRXI, TDPX2)
  • Tags / Fusion Proteins
    • His6-TEV (N terminal on insert)
    • AviTag (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2-pBirA cells for expression.

Please note: Plasmid is missing the GSGGGGSGGGGS linker sequence compared to the depositor's sequence. The missing extra linker between the full length coding sequence and Avitag does not affect function of the proteins.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PRDX1A-c001 was a gift from Opher Gileadi (Addgene plasmid # 175473 ; http://n2t.net/addgene:175473 ; RRID:Addgene_175473)
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