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Addgene

MDKA-c009
(Plasmid #175463)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHL-Sec
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MDK
  • Alt name
    ARAP|MK|NEGF2
  • Species
    H. sapiens (human)
  • Entrez Gene
    MDK (a.k.a. ARAP, MK, NEGF2)
  • Tags / Fusion Proteins
    • PTPRS (Gg) SP (N terminal on insert)
    • His6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gileadi lab uses HEK293 cells for expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MDKA-c009 was a gift from Opher Gileadi (Addgene plasmid # 175463 ; http://n2t.net/addgene:175463 ; RRID:Addgene_175463)
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