EPB41L3A-c021
(Plasmid
#175460)
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PurposeProtein expression in bacterial cells. FERM domain, P107-L390. Used for crystallography (PDB:6IBE).
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175460 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNIC28-Bsa4
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEPB41L3
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Alt name4.1B|DAL-1|DAL1
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SpeciesH. sapiens (human)
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Entrez GeneEPB41L3 (a.k.a. 4.1B, DAL-1, DAL1)
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Tag
/ Fusion Protein
- His6-TEV (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EPB41L3A-c021 was a gift from Opher Gileadi (Addgene plasmid # 175460 ; http://n2t.net/addgene:175460 ; RRID:Addgene_175460)