CD44MMA-c002
(Plasmid
#175452)
-
PurposeProtein expression in bacterial cells (as inclusion bodies). CD44 Link module, Q23-D172. Used for crystallography.
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 175452 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepNIC-CTH0
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCd44
-
Alt nameLy-24|Pgp-1| HERMES|AU023126|AW121933|AW146109
-
SpeciesM. musculus (mouse)
-
Entrez GeneCd44 (a.k.a. HERMES, Ly-24, Pgp-1)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
CD44MMA-c002 was a gift from Opher Gileadi (Addgene plasmid # 175452 ; http://n2t.net/addgene:175452 ; RRID:Addgene_175452)