pAAV-VTKS5-TVA-eGFP-N2cG
(Plasmid
#175440)
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PurposeCre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175440 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-VTKS5
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Backbone manufacturerJordane Dimidschstein (Addgene plasmid # 170856)
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Vector typeAAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTVA-P2A-eGFP-P2A-N2cG
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-VTKS5-TVA-eGFP-N2cG was a gift from Gordon Fishell (Addgene plasmid # 175440 ; http://n2t.net/addgene:175440 ; RRID:Addgene_175440) -
For your References section:
The organization and development of cortical interneuron presynaptic circuits are area specific. Pouchelon G, Dwivedi D, Bollmann Y, Agba CK, Xu Q, Mirow AMC, Kim S, Qiu Y, Sevier E, Ritola KD, Cossart R, Fishell G. Cell Rep. 2021 Nov 9;37(6):109993. doi: 10.1016/j.celrep.2021.109993. 10.1016/j.celrep.2021.109993 PubMed 34758329