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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17543 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTYB3
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Backbone manufacturerNew England Biolabs
- Backbone size w/o insert (bp) 7477
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehuman PUM1 Pumilio homology domain
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Alt namePUM1
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SpeciesH. sapiens (human)
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Insert Size (bp)1053
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Entrez GenePUM1 (a.k.a. HSPUM, NEDMSF, PUMH, PUMH1, PUML1, SCA47)
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Tag
/ Fusion Protein
- intein (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site SapI (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer IntR (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTYB3-PUM1-HD was a gift from Traci Hall (Addgene plasmid # 17543 ; http://n2t.net/addgene:17543 ; RRID:Addgene_17543) -
For your References section:
Crystal structure of a Pumilio homology domain. Wang X, Zamore PD, Hall TM. Mol Cell. 2001 Apr . 7(4):855-65. 10.1016/S1097-2765(01)00229-5 PubMed 11336708