pCDNA4TO-3XFLAG Halo R124A/K125A nsp1
(Plasmid #175417)

• Purpose: Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.
• Depositing Lab: Britt Glaunsinger
• Publication: Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi: 10.1016/j.celrep.2021.109841. Epub 2021 Sep 30.

Backbone

• Vector backbone: pCDNA4TO
• Backbone size w/o insert (bp): 6028
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CoV2 R124A/K125A nsp1
• Alt name: nsp1 RNase destabilization mutant
• Species: CoV2
• Insert Size (bp): 539
• Mutation: R124A and K125A
• Promoter: CMV
• Tag / Fusion Protein:
  • 3XFLAG Halo (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry