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Addgene

Sc NybbR eRF1 (G180A)
(Plasmid #175415)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175415 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    1C
  • Backbone manufacturer
    UC Berkeley MacroLab
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eRF1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    G180A
  • Entrez Gene
    SUP45 (a.k.a. YBR143C, SAL4, SUP1, SUP47)
  • Promoter T7
  • Tags / Fusion Proteins
    • His-MBP-TEV (N terminal on backbone)
    • ybbR (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sc NybbR eRF1 (G180A) was a gift from Joseph Puglisi (Addgene plasmid # 175415 ; http://n2t.net/addgene:175415 ; RRID:Addgene_175415)

  • For your References section:

    Mechanisms that ensure speed and fidelity in eukaryotic translation termination. Lawson MR, Lessen LN, Wang J, Prabhakar A, Corsepius NC, Green R, Puglisi JD. Science. 2021 Aug 20;373(6557):876-882. doi: 10.1126/science.abi7801. 10.1126/science.abi7801 PubMed 34413231
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