RS314-H
(Plasmid #17541)

• Depositing Lab: Bert Vogelstein
• Publication: Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 92(13):6062-6.

Backbone

• Vector backbone: pRS314
• Backbone size w/o insert (bp): 4800
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Gal1-p53
• Alt name: p53
• Species: H. sapiens (human)
• Mutation: R273H
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

RS314-H contains p53 with the following mutation: R273H.