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Addgene

pDN625
(Plasmid #175379)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175379 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDN625
  • Backbone size (bp) 6914
  • Vector type
    Yeast Expression, Cre/Lox
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was generated by modification of plasmid pRS405 (Sikorski & Hieter, 1989, Genetics 122:19-27) by insertion of 2 micron high copy S. cerevisiae replication and partitioning locus with flanking restriction enzyme cut sites (AatII) and flanking LoxP sites.

bioRxiv pre-print 367219: Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
https://doi.org/10.1101/2020.11.03.367219

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDN625 was a gift from Daniel Nickerson (Addgene plasmid # 175379 ; http://n2t.net/addgene:175379 ; RRID:Addgene_175379)

  • For your References section:

    Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase. Nickerson DP, Quinn MA, Milnes JM. G3 (Bethesda). 2021 Dec 8;11(12). pii: 6380430. doi: 10.1093/g3journal/jkab336. 10.1093/g3journal/jkab336 PubMed 34599813
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