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Addgene

pDN514
(Plasmid #175366)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175366 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDN514
  • Backbone size (bp) 4838
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was generated by modification of plasmid pRS404 (Sikorski & Hieter, 1989, Genetics 122:19-27) by insertion of CEN6/ARSH4 low copy S. cerevisiae replication and partitioning locus with flanking restriction enzyme cut sites (AatII, AvrII, and SphI).

bioRxiv pre-print 367219: Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
https://doi.org/10.1101/2020.11.03.367219

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDN514 was a gift from Daniel Nickerson (Addgene plasmid # 175366 ; http://n2t.net/addgene:175366 ; RRID:Addgene_175366)

  • For your References section:

    Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase. Nickerson DP, Quinn MA, Milnes JM. G3 (Bethesda). 2021 Dec 8;11(12). pii: 6380430. doi: 10.1093/g3journal/jkab336. 10.1093/g3journal/jkab336 PubMed 34599813
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