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Addgene

anti-FLAG M2 heavy chain
(Plasmid #175359)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175359 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-FLAG M2 heavy chain
  • Alt name
    D55
  • Species
    M. musculus (mouse)
  • Mutation
    L51I (see paper)
  • Promoter CMV
  • Tags / Fusion Proteins
    • His8 tag (C terminal on insert)
    • N-terminal secretion signal peptide (MEAPAQLLFLLLLWLPDTTG) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    anti-FLAG M2 heavy chain was a gift from Joost Snijder (Addgene plasmid # 175359 ; http://n2t.net/addgene:175359 ; RRID:Addgene_175359)

  • For your References section:

    Mass Spectrometry-Based De Novo Sequencing of Monoclonal Antibodies Using Multiple Proteases and a Dual Fragmentation Scheme. Peng W, Pronker MF, Snijder J. J Proteome Res. 2021 Jul 2;20(7):3559-3566. doi: 10.1021/acs.jproteome.1c00169. Epub 2021 Jun 14. 10.1021/acs.jproteome.1c00169 PubMed 34121409
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