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Addgene

pT7RNAP-Q737P
(Plasmid #175356)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175356 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCK01
  • Backbone size w/o insert (bp) 3095
  • Total vector size (bp) 5693
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    T7RNAP-Q737P
  • Species
    T7 Phage
  • Insert Size (bp)
    2798
  • Mutation
    Q737P

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7RNAP-Q737P was a gift from Sven Panke (Addgene plasmid # 175356 ; http://n2t.net/addgene:175356 ; RRID:Addgene_175356)

  • For your References section:

    Plasmid replication based on the T7 origin of replication requires a T7 RNAP variant and inactivation of ribonuclease H. Becker K, Meyer A, Roberts TM, Panke S. Nucleic Acids Res. 2021 Jul 13. pii: 6320377. doi: 10.1093/nar/gkab596. 10.1093/nar/gkab596 PubMed 34255845
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