pT7RNAP-Q737P
(Plasmid
#175356)
-
PurposeSupport plasmid for T7_ori plasmids
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175356 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCK01
- Backbone size w/o insert (bp) 3095
- Total vector size (bp) 5693
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameT7RNAP-Q737P
-
SpeciesT7 Phage
-
Insert Size (bp)2798
-
MutationQ737P
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pT7RNAP-Q737P was a gift from Sven Panke (Addgene plasmid # 175356 ; http://n2t.net/addgene:175356 ; RRID:Addgene_175356) -
For your References section:
Plasmid replication based on the T7 origin of replication requires a T7 RNAP variant and inactivation of ribonuclease H. Becker K, Meyer A, Roberts TM, Panke S. Nucleic Acids Res. 2021 Jul 13. pii: 6320377. doi: 10.1093/nar/gkab596. 10.1093/nar/gkab596 PubMed 34255845
