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Addgene

pH6HTC_MANF-Halo
(Plasmid #175341)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175341 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pH6HTC_minus_cer-region
  • Vector type
    Bacterial Expression ; Cell Free Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MANF
  • Species
    H. sapiens (human)
  • Entrez Gene
    MANF (a.k.a. ARMET, ARP, DDDS)
  • Tag / Fusion Protein
    • HaloTag-6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.11.08.467743 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH6HTC_MANF-Halo was a gift from Claudia Kutter (Addgene plasmid # 175341 ; http://n2t.net/addgene:175341 ; RRID:Addgene_175341)

  • For your References section:

    Large-scale identification of RBP-RNA interactions by RAPseq refines essentials of post-transcriptional gene regulation. Atanasoai I, Papavasileiou S, Preiß N, Kutter C. bioRxiv 2021.11.08.467743 10.1101/2021.11.08.467743
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