pH6HTC_ENO1-Halo
(Plasmid #175330)

• Purpose: Production of HaloTag fusion protein
• Depositing Lab: Claudia Kutter
• Publication: Atanasoai et al bioRxiv 2021.11.08.467743

Backbone

• Vector backbone: pH6HTC_minus_cer-region
• Vector type: Bacterial Expression ; Cell Free Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ENO1
• Species: H. sapiens (human)
• Entrez Gene: ENO1 (a.k.a. ENO1-IT1, ENO1L1, HEL-S-17, MPB1, NNE, PPH)
• Tag / Fusion Protein:
  • HaloTag-6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note in Addgene's NGS result identified a single ambiguous base in the insert adjacent to the TEV site. This base is a mixed population of A (48.4%) and G (51.2%). If it is an A the amino acid changes from G to E. Depositor confirmed this mutation does not affect plasmid function. Please visit https://doi.org/10.1101/2021.11.08.467743 for bioRxiv preprint.