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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17532 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1 (Clontech)
- Backbone size w/o insert (bp) 3417
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVSV-G glycoprotein (Indiana strain)
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Speciesvesicular stomatitis virus
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Insert Size (bp)2415
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GenBank IDJ02428
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Use packaging construct encoding Tat
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLTR-G was a gift from Jakob Reiser (Addgene plasmid # 17532 ; http://n2t.net/addgene:17532 ; RRID:Addgene_17532) -
For your References section:
Transduction of nondividing cells using pseudotyped defective high-titer HIV type 1 particles. Reiser J, Harmison G, Kluepfel-Stahl S, Brady RO, Karlsson S, Schubert M. Proc Natl Acad Sci U S A. 1996 Dec 24. 93(26):15266-71. 10.1073/pnas.93.26.15266 PubMed 8986799