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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17531 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 (Invitrogen)
- Backbone size w/o insert (bp) 2246
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHIV-1 Gag/Pol, Tat, Rev
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SpeciesHIV-1
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Insert Size (bp)7274
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCD/NL-BH*DDD was a gift from Jakob Reiser (Addgene plasmid # 17531 ; http://n2t.net/addgene:17531 ; RRID:Addgene_17531) -
For your References section:
Transduction of bone-marrow-derived mesenchymal stem cells by using lentivirus vectors pseudotyped with modified RD114 envelope glycoproteins. Zhang XY, La Russa VF, Reiser J. J Virol. 2004 Feb . 78(3):1219-29. 10.1128/JVI.78.3.1219-1229.2004 PubMed 14722277