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Addgene

pPGK-loxP-neoEGFP (plox)
(Plasmid #17530)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17530 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pPGK-loxP-neoEGFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI, EcoRI, MluI, KpnI (not destroyed)
  • 3′ cloning site NotI, XbaI, SalI, SmaI, NotI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The lox-targeting plasmid, plox, was generated by subcloning the Sal I (blunted)-Hind III fragment of pPGK-loxP-Xist (Wutz et al., 2002) into Bgl II (blunted)-Hind III cut pNeoEGFP (Clontech). plox has a stuffer fragment (the EGFP gene) derived from pNeoEGFP, bounded by multiple cloning sites upstream and downstream.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPGK-loxP-neoEGFP (plox) was a gift from George Daley (Addgene plasmid # 17530)

  • For your References section:

    HoxB4 confers definitive lymphoid-myeloid engraftment potential on embryonic stem cell and yolk sac hematopoietic progenitors. Kyba M, Perlingeiro RC, Daley GQ. Cell. 2002 Apr 5. 109(1):29-37. 10.1016/S0092-8674(02)00680-3 PubMed 11955444
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