pPGK-loxP-neoEGFP (plox)
(Plasmid
#17530)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17530 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPGK-loxP-neoEGFP
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI, EcoRI, MluI, KpnI (not destroyed)
- 3′ cloning site NotI, XbaI, SalI, SmaI, NotI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The lox-targeting plasmid, plox, was generated by subcloning the Sal I (blunted)-Hind III fragment of pPGK-loxP-Xist (Wutz et al., 2002) into Bgl II (blunted)-Hind III cut pNeoEGFP (Clontech). plox has a stuffer fragment (the EGFP gene) derived from pNeoEGFP, bounded by multiple cloning sites upstream and downstream.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPGK-loxP-neoEGFP (plox) was a gift from George Daley (Addgene plasmid # 17530) -
For your References section:
HoxB4 confers definitive lymphoid-myeloid engraftment potential on embryonic stem cell and yolk sac hematopoietic progenitors. Kyba M, Perlingeiro RC, Daley GQ. Cell. 2002 Apr 5. 109(1):29-37. 10.1016/S0092-8674(02)00680-3 PubMed 11955444
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/30/27/2abb3ec4-af62-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)