pH6HTC_minus_cer-region
(Plasmid
#175297)
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Purpose(Empty Backbone) Production of HaloTag fusion protein
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175297 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepH6HTC
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Vector typeBacterial Expression ; Cell Free Expression
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Tag
/ Fusion Protein
- HaloTag-6xHis (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- HaloTag-6xHis (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.11.08.467743 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pH6HTC_minus_cer-region was a gift from Claudia Kutter (Addgene plasmid # 175297 ; http://n2t.net/addgene:175297 ; RRID:Addgene_175297) -
For your References section:
Large-scale identification of RBP-RNA interactions by RAPseq refines essentials of post-transcriptional gene regulation. Atanasoai I, Papavasileiou S, Preiß N, Kutter C. bioRxiv 2021.11.08.467743 10.1101/2021.11.08.467743
