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Addgene

pCMV-Frankenbody-FLAG-mRuby2
(Plasmid #175294)

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Item Catalog # Description Quantity Price (USD)
Plasmid 175294 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pmCherry-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-FLAG frankenbody fused with mRuby2
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • mRuby2 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Read the preprint at bioRxiv: doi.org/10.1101/2021.06.16.448697.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Frankenbody-FLAG-mRuby2 was a gift from Tim Stasevich (Addgene plasmid # 175294 ; http://n2t.net/addgene:175294 ; RRID:Addgene_175294)

  • For your References section:

    Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells. Liu Y, Zhao N, Kanemaki MT, Yamamoto Y, Sadamura Y, Ito Y, Tokunaga M, Stasevich TJ, Kimura H. Genes Cells. 2021 Nov;26(11):905-926. doi: 10.1111/gtc.12893. Epub 2021 Sep 20. 10.1111/gtc.12893 PubMed 34465007
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