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Addgene

AAV-Syn-NS1
(Plasmid #175276)

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Item Catalog # Description Quantity Price (USD)
Plasmid 175276 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV2
  • Backbone size w/o insert (bp) 4591
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato
  • Species
    Yellow fever virus strain 17D
  • Insert Size (bp)
    1922
  • Entrez Gene
    POLY (a.k.a. YFVgp1)
  • Promoter Synapsin
  • Tag / Fusion Protein
    • NLS-dTomato (P2A cleavage) (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer actcagcgctgcctcagtc
  • 3′ sequencing primer CTCCTCATAAAGAGACAGCAACC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-Syn-NS1 was a gift from Wei Xu (Addgene plasmid # 175276 ; http://n2t.net/addgene:175276 ; RRID:Addgene_175276)

  • For your References section:

    Anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine YFV-17D. Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W. Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 10.1038/s41592-021-01319-9. 10.1038/s41592-021-01319-9 PubMed 34824475
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