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Addgene

ALPaGA
(Plasmid #175272)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175272 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB4K5
  • Backbone size w/o insert (bp) 3416
  • Total vector size (bp) 5603
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LldR_Synthetic_promoter_sfGFP
  • Species
    Synthetic; E. coli
  • Insert Size (bp)
    2227

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ctcggatacccttactctgttgaaaacg
  • 3′ sequencing primer TCTTACTGAAGCACGATTCTACTCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ALPaGA was a gift from Jerome Bonnet (Addgene plasmid # 175272 ; http://n2t.net/addgene:175272 ; RRID:Addgene_175272)

  • For your References section:

    Engineered l-Lactate Responding Promoter System Operating in Glucose-Rich and Anoxic Environments. Zuniga A, Camacho M, Chang HJ, Fristot E, Mayonove P, Hani EH, Bonnet J. ACS Synth Biol. 2021 Dec 1. doi: 10.1021/acssynbio.1c00456. 10.1021/acssynbio.1c00456 PubMed 34851606
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