pPB-EF1a-MegaGate-DD-Blast
(Plasmid
#175271)
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Purpose(Empty Backbone) MegaGate destination with C-terminal DD tag and blasticidin resistance
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175271 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePiggyBac
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Vector typeMammalian Expression
- Promoter EF1a
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Selectable markersBlasticidin
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Tag
/ Fusion Protein
- Destabilization Domain (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-fwd
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Selectable marker is flanked by Rox sites for excision with Dre recombinase.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-EF1a-MegaGate-DD-Blast was a gift from George Church (Addgene plasmid # 175271 ; http://n2t.net/addgene:175271 ; RRID:Addgene_175271) -
For your References section:
An integrated pipeline for mammalian genetic screening. Kramme C, Plesa AM, Wang HH, Wolf B, Smela MP, Guo X, Kohman RE, Chatterjee P, Church GM. Cell Rep Methods. 2021 Sep 27;1(6):100082. doi: 10.1016/j.crmeth.2021.100082. eCollection 2021 Oct 25. 10.1016/j.crmeth.2021.100082 PubMed 35474898
