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Addgene

pBRIT Pax7 N215-273C
(Plasmid #17527)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17527 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBRIT-LoxP-HA/FLAG-puro
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression, Retroviral, Cre/Lox
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pax7
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1300
  • Mutation
    Deletion of aa 216-272 (homeodomain)
  • Entrez Gene
    Pax7 (a.k.a. Pax-7)
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pBABE5'
  • 3′ sequencing primer pBABE3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBRIT Pax7 N215-273C was a gift from Michael Rudnicki (Addgene plasmid # 17527 ; http://n2t.net/addgene:17527 ; RRID:Addgene_17527)

  • For your References section:

    Pax7 activates myogenic genes by recruitment of a histone methyltransferase complex. McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, Greenblatt JF, Dilworth FJ, Rudnicki MA. Nat Cell Biol. 2008 Jan . 10(1):77-84. 10.1038/ncb1671 PubMed 18066051
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