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Addgene

pPB-EF1a-MegaGate-DD-Hygro
(Plasmid #175267)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175267 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    PiggyBac
  • Vector type
    Mammalian Expression
  • Promoter EF1a
  • Selectable markers
    Hygromycin
  • Tag / Fusion Protein
    • Destabilization Domain (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-EF1a-MegaGate-DD-Hygro was a gift from George Church (Addgene plasmid # 175267 ; http://n2t.net/addgene:175267 ; RRID:Addgene_175267)
  • For your References section:

    An integrated pipeline for mammalian genetic screening. Kramme C, Plesa AM, Wang HH, Wolf B, Smela MP, Guo X, Kohman RE, Chatterjee P, Church GM. Cell Rep Methods. 2021 Sep 27;1(6):100082. doi: 10.1016/j.crmeth.2021.100082. eCollection 2021 Oct 25. 10.1016/j.crmeth.2021.100082 PubMed 35474898