pTub-alpha-EB3-NeonGreen
(Plasmid #175263)

• Purpose: labelling of microtubule plus end and F-actin structures
• Depositing Labs: Frank Bradke, Sebastián Dupraz
• Publication: Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.3791/63068.

Backbone

• Vector backbone: pTub-alpha1-ZsGreen-N1
• Backbone size w/o insert (bp): 4495
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EB3
• Species: H. sapiens (human)
• Insert Size (bp): 1569
• Mutation: None (wt)
• Entrez Gene: MAPRE3 (a.k.a. EB3, EBF3, EBF3-S, RP3)
• Promoter: pTub-alpha1
• Tag / Fusion Protein:
  • mNeonGreen (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pTub-alpha1 ( 5'-GTAAGAAGCAACACCTCCTC-3')
• 3′ sequencing primer: NeonGFP reverse (5’-GGAGAGAGGCCATGTTATCC-3’)

Resource Information

• Supplemental Documents:
  • pTub-alpha1-EB3-mNeonGreen.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry