EGFP-SPN_ARR wt (Shank3)
(Plasmid
#175254)
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PurposeSPN-ARR fragment in EGFP backbone for mammalian expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175254 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSPN-ARR fragment from SHANK3
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-SPN_ARR wt (Shank3) was a gift from Johanna Ivaska (Addgene plasmid # 175254 ; http://n2t.net/addgene:175254 ; RRID:Addgene_175254) -
For your References section:
SHANK3 conformation regulates direct actin binding and crosstalk with Rap1 signaling. Salomaa SI, Miihkinen M, Kremneva E, Paatero I, Lilja J, Jacquemet G, Vuorio J, Antenucci L, Kogan K, Hassani Nia F, Hollos P, Isomursu A, Vattulainen I, Coffey ET, Kreienkamp HJ, Lappalainen P, Ivaska J. Curr Biol. 2021 Sep 29. pii: S0960-9822(21)01262-8. doi: 10.1016/j.cub.2021.09.022. 10.1016/j.cub.2021.09.022 PubMed 34610274
