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Addgene

Cbrg-vab-1-RNAi
(Plasmid #175249)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175249 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    L4400
  • Total vector size (bp) 5007
  • Vector type
    RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli HT115
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    vab-1
  • Alt name
    CBG13447
  • Species
    Caenorhabditis briggsae
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cbrg-vab-1-RNAi was a gift from Anne Brunet (Addgene plasmid # 175249 ; http://n2t.net/addgene:175249 ; RRID:Addgene_175249)

  • For your References section:

    Self-sperm induce resistance to the detrimental effects of sexual encounters with males in hermaphroditic nematodes. Booth LN, Maures TJ, Yeo RW, Tantilert C, Brunet A. Elife. 2019 Jul 8;8. pii: 46418. doi: 10.7554/eLife.46418. 10.7554/eLife.46418 PubMed 31282863
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