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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBRIT-LoxP-TAP-puro
- Backbone size (bp) 5200
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Vector typeMammalian Expression, Retroviral, Cre/Lox
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- TAP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
TAP tag is 6xHis-TEV-3xFLAG (note that two of the FLAG tags are not canonical--the amino acid sequence of the 3xFLAG region is: DYKDHDGDYKDHDIDYKDDDDK)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBRIT TAP was a gift from Michael Rudnicki (Addgene plasmid # 17520 ; http://n2t.net/addgene:17520 ; RRID:Addgene_17520) -
For your References section:
Pax7 activates myogenic genes by recruitment of a histone methyltransferase complex. McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, Greenblatt JF, Dilworth FJ, Rudnicki MA. Nat Cell Biol. 2008 Jan . 10(1):77-84. 10.1038/ncb1671 PubMed 18066051