pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA
(Plasmid #175177)

• Purpose: Cre-dependent GCaMP6s and mBeRFP expression
• Depositing Lab: Bernardo Sabatini
• Publication: Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25. doi: 10.1016/j.cell.2021.09.013. Epub 2021 Oct 4.

Backbone

• Vector backbone: pAAV
• Total vector size (bp): 7116
• Modifications to backbone: Kozak sequence followed by GCaMP6s-GSG-P2A-mBeRFP inserted in between loxP sites in reverse reading direction, driven by CAG promoter
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GCaMP6s
• Insert Size (bp): 3210
• Promoter: CAG
• Tag / Fusion Protein:
  • P2A-mBeRFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pAAV-CBA-DIO-GCaMP6s-mBeRFP_corrected.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mBeRFP sequence is based on the sequences and mutations described in Yang et al., 2013: Yang, J., Wang, L., Yang, F., Luo, H., Xu, L., Lu, J., Zeng, S., and Zhang, Z. (2013). mBeRFP, an improved large stokes shift red fluorescent protein. PLoS One 8.