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Addgene

pLV CMV IGF1-FLAG
(Plasmid #175170)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175170 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-CMV-GFP-Puro
  • Backbone manufacturer
    Eric Campeau & Paul Kaufman (Addgene plasmid # 17448)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Igf1
  • Species
    H. sapiens (human)
  • Entrez Gene
    IGF1 (a.k.a. IGF, IGF-I, IGFI, MGF)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IGF-I from Dugay and Steiner, JBC 270:17566-74 (1995)

Encodes epitope-tagged human Pre-pro-IGF1 as described in JBC 270:17566-74 (1995), with an FLAG tag inserted between the “pre” signal sequence and before the pro-IGF1 sequence. The FLAG tag becomes N-terminal after cleavage of the signal sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV CMV IGF1-FLAG was a gift from Larry Gerace (Addgene plasmid # 175170 ; http://n2t.net/addgene:175170 ; RRID:Addgene_175170)
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