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Addgene

pLV CDHblast Rev(V16D)-EGFP-V5
(Plasmid #175165)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175165 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDHblast-MCSNard OST-LMNA
  • Backbone manufacturer
    Tom Misteli (Addgene plasmid # 22661)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rev
  • Species
    HIV-1
  • Mutation
    V16D
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV CDHblast Rev(V16D)-EGFP-V5 was a gift from Larry Gerace (Addgene plasmid # 175165 ; http://n2t.net/addgene:175165 ; RRID:Addgene_175165)

  • For your References section:

    A Survey of DDX21 Activity During Rev/RRE Complex Formation. Hammond JA, Zhou L, Lamichhane R, Chu HY, Millar DP, Gerace L, Williamson JR. J Mol Biol. 2018 Feb 16;430(4):537-553. doi: 10.1016/j.jmb.2017.06.023. Epub 2017 Jul 10. 10.1016/j.jmb.2017.06.023 PubMed 28705764
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