pLV CMV mCherry-DDX1-V5
(Plasmid
#175162)
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PurposeLentiviral expression of human DDX1-V5
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV-CMV-GFP-Puro
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Backbone manufacturerEric Campeau & Paul Kaufman (Addgene plasmid # 17448)
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDDX1
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SpeciesH. sapiens (human)
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Entrez GeneDDX1 (a.k.a. DBP-RB, UKVH5d)
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV CMV mCherry-DDX1-V5 was a gift from Larry Gerace (Addgene plasmid # 175162 ; http://n2t.net/addgene:175162 ; RRID:Addgene_175162) -
For your References section:
A Survey of DDX21 Activity During Rev/RRE Complex Formation. Hammond JA, Zhou L, Lamichhane R, Chu HY, Millar DP, Gerace L, Williamson JR. J Mol Biol. 2018 Feb 16;430(4):537-553. doi: 10.1016/j.jmb.2017.06.023. Epub 2017 Jul 10. 10.1016/j.jmb.2017.06.023 PubMed 28705764