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Addgene

pLV Tmem53-V5
(Plasmid #175142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-EF1a-IRES-Puro
  • Backbone manufacturer
    Tobias Meyer (Addgene plasmid # 85132)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tmem53
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tmem53 (a.k.a. 1110038M16Rik, 1500001P22Rik, 1700012A05Rik)
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Uniprot ID Q9D0Z3-1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV Tmem53-V5 was a gift from Larry Gerace (Addgene plasmid # 175142 ; http://n2t.net/addgene:175142 ; RRID:Addgene_175142)

  • For your References section:

    Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelope. Cheng LC, Zhang X, Baboo S, Nguyen JA, Martinez-Bartolome S, Loose E, Diedrich J, Yates JR 3rd, Gerace L. Life Sci Alliance. 2023 Jul 11;6(9):e202301998. doi: 10.26508/lsa.202301998. Print 2023 Sep. 10.26508/lsa.202301998 PubMed 37433644
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