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Addgene

pLV Tmx4-V5 C209A, C211A
(Plasmid #175141)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175141 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLV-EF1a-IRES-Puro
  • Backbone manufacturer
    Tobias Meyer (Addgene plasmid # 85132)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tmx4
  • Alt name
    D2Bwg1356e
  • Alt name
    Kiaa1162
  • Species
    M. musculus (mouse)
  • Mutation
    C209A, C211A
  • Entrez Gene
    Tmx4 (a.k.a. 2810417D04Rik, 4930500L08Rik, AI843224, AW046784, D2Bwg1356e, Txndc13, mKIAA1162)
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV Tmx4-V5 C209A, C211A was a gift from Larry Gerace (Addgene plasmid # 175141 ; http://n2t.net/addgene:175141 ; RRID:Addgene_175141)
  • For your References section:

    Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelope. Cheng LC, Zhang X, Baboo S, Nguyen JA, Martinez-Bartolome S, Loose E, Diedrich J, Yates JR 3rd, Gerace L. Life Sci Alliance. 2023 Jul 11;6(9):e202301998. doi: 10.26508/lsa.202301998. Print 2023 Sep. 10.26508/lsa.202301998 PubMed 37433644