pLV Tmx4-V5 C209A, C211A
(Plasmid
#175141)
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PurposeLentiviral expression of V5-tagged mouse Tmx4
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV-EF1a-IRES-Puro
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Backbone manufacturerTobias Meyer (Addgene plasmid # 85132)
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTmx4
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Alt nameD2Bwg1356e
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Alt nameKiaa1162
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SpeciesM. musculus (mouse)
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MutationC209A, C211A
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Entrez GeneTmx4 (a.k.a. 2810417D04Rik, 4930500L08Rik, AI843224, AW046784, D2Bwg1356e, Txndc13, mKIAA1162)
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV Tmx4-V5 C209A, C211A was a gift from Larry Gerace (Addgene plasmid # 175141 ; http://n2t.net/addgene:175141 ; RRID:Addgene_175141) -
For your References section:
Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelope. Cheng LC, Zhang X, Baboo S, Nguyen JA, Martinez-Bartolome S, Loose E, Diedrich J, Yates JR 3rd, Gerace L. Life Sci Alliance. 2023 Jul 11;6(9):e202301998. doi: 10.26508/lsa.202301998. Print 2023 Sep. 10.26508/lsa.202301998 PubMed 37433644