pLV Reep4-V5
(Plasmid
#175120)
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PurposeLentiviral expression of mouse Reep4-V5
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175120 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV-EF1a-IRES-Puro
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Backbone manufacturerTobias Meyer (Addgene plasmid # 85132)
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameReep4
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SpeciesM. musculus (mouse)
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Entrez GeneReep4 (a.k.a. 2700029E10Rik)
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV Reep4-V5 was a gift from Larry Gerace (Addgene plasmid # 175120 ; http://n2t.net/addgene:175120 ; RRID:Addgene_175120) -
For your References section:
Shared and Distinctive Neighborhoods of Emerin and Lamin B Receptor Revealed by Proximity Labeling and Quantitative Proteomics. Cheng LC, Zhang X, Abhinav K, Nguyen JA, Baboo S, Martinez-Bartolome S, Branon TC, Ting AY, Loose E, Yates JR 3rd, Gerace L. J Proteome Res. 2022 Sep 2;21(9):2197-2210. doi: 10.1021/acs.jproteome.2c00281. Epub 2022 Aug 16. 10.1021/acs.jproteome.2c00281 PubMed 35972904