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Addgene

pLV V5-TbID-emerin
(Plasmid #175099)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175099 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-EF1a-IRES-Puro
  • Backbone manufacturer
    Tobias Meyer (Addgene plasmid # 85132)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Emerin
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Emd (a.k.a. AW550900, Sta)
  • Tag / Fusion Protein
    • V5-TbID (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TbID, TurboID: Nat Biotechnol. (2018) 36:880-887

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV V5-TbID-emerin was a gift from Larry Gerace (Addgene plasmid # 175099 ; http://n2t.net/addgene:175099 ; RRID:Addgene_175099)

  • For your References section:

    Shared and Distinctive Neighborhoods of Emerin and Lamin B Receptor Revealed by Proximity Labeling and Quantitative Proteomics. Cheng LC, Zhang X, Abhinav K, Nguyen JA, Baboo S, Martinez-Bartolome S, Branon TC, Ting AY, Loose E, Yates JR 3rd, Gerace L. J Proteome Res. 2022 Sep 2;21(9):2197-2210. doi: 10.1021/acs.jproteome.2c00281. Epub 2022 Aug 16. 10.1021/acs.jproteome.2c00281 PubMed 35972904
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