pEF6 Vps54-V5-6His
(Plasmid
#175098)
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PurposeV5 tagged Vps54 is expressed in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175098 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEF6-V5-His
- Backbone size w/o insert (bp) 5840
- Total vector size (bp) 8735
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVps54
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SpeciesM. musculus (mouse)
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Insert Size (bp)2895
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Entrez GeneVps54 (a.k.a. Hcc8, Vps54l, mSLP8, wr)
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF6 Vps54-V5-6His was a gift from Juan Bonifacino (Addgene plasmid # 175098 ; http://n2t.net/addgene:175098 ; RRID:Addgene_175098) -
For your References section:
Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Perez-Victoria FJ, Mardones GA, Bonifacino JS. Mol Biol Cell. 2008 Jun;19(6):2350-62. doi: 10.1091/mbc.e07-11-1189. Epub 2008 Mar 26. 10.1091/mbc.e07-11-1189 PubMed 18367545
