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Addgene

pmEGFP-N1 SYS1
(Plasmid #175092)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175092 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 4716
  • Total vector size (bp) 5184
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SYS1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    468
  • GenBank ID
    NM_001197129.2 NM_033542.4
  • Entrez Gene
    SYS1 (a.k.a. C20orf169, dJ453C12.4, dJ453C12.4.1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmEGFP-N1 SYS1 was a gift from Juan Bonifacino (Addgene plasmid # 175092 ; http://n2t.net/addgene:175092 ; RRID:Addgene_175092)

  • For your References section:

    ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network. Ishida M, Bonifacino JS. J Cell Biol. 2019 Nov 4;218(11):3681-3696. doi: 10.1083/jcb.201905097. Epub 2019 Oct 1. 10.1083/jcb.201905097 PubMed 31575603
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