Skip to main content
Addgene

pET16better_H10_HRV3C_chitinase
(Plasmid #175091)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175091 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET-16b
  • Backbone manufacturer
    Novagen (EMD Millipore)
  • Backbone size w/o insert (bp) 5651
  • Total vector size (bp) 7442
  • Modifications to backbone
    Factor Xa cleavage site replaced by HRV-3C cleavage site
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    chitinase
  • Species
    B. licheniformis
  • Insert Size (bp)
    1866
  • Tag / Fusion Protein
    • 10X His, HRV-3C (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET16better_H10_HRV3C_chitinase was a gift from Jay C Dunlap (Addgene plasmid # 175091 ; http://n2t.net/addgene:175091 ; RRID:Addgene_175091)

  • For your References section:

    Quantitative single molecule RNA-FISH and RNase-free cell wall digestion in Neurospora crassa. Bartholomai BM, Gladfelter AS, Loros JJ, Dunlap JC. Fungal Genet Biol. 2021 Aug 20;156:103615. doi: 10.1016/j.fgb.2021.103615. 10.1016/j.fgb.2021.103615 PubMed 34425213
Related items:
Commonly requested with: